Pectobacterium carotovorum subsp. carotovorum - the causal agent of broccoli soft rot in Serbia
Soft rot symptoms were observed on broccoli plants in several commercial fields in the western part of Serbia. Six strains of bacteria were isolated from diseased tissues and identified as Pectobacterium carotovorum subsp. carotovorum using conventional bacteriological and molecular methods. All str...
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Published in | Pesticidi i Fitomedicina Vol. 29; no. 4; pp. 249 - 255 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Institute of Pesticides and Environmental Protection
01.12.2014
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Subjects | |
Online Access | Get full text |
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Summary: | Soft rot symptoms were observed on broccoli plants in several commercial
fields in the western part of Serbia. Six strains of bacteria were isolated
from diseased tissues and identified as Pectobacterium carotovorum subsp.
carotovorum using conventional bacteriological and molecular methods. All
strains were non-fluorescent, gram-negative, facultative anaerobes,
oxidase-negative and catalase-positive, causing soft rot on potato and carrot
slices and did not induce hypersensitive reaction on tobacco leaves. They
grew in 5% NaCl and at 37?C, did not produce acid from ?-methyl glucoside,
sorbitol and maltose, nor reducing substances from sucrose, but utilized
lactose and trehalose, and did not produce indole or lecithinase. The
investigated strains showed characteristic growth on Logan?s medium and did
not produce blue pigmented indigoidine on GYCA medium nor ?fried egg?
colonies on PDA. The identity of strains was confirmed by ITS-PCR and
ITS-RFLP analyses and by sequence analysis of the 16S rRNA gene. In a
pathogenicity assay, all strains caused tissue discoloration and soft rot
development on inoculated broccoli head tissue fragments. |
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ISSN: | 1820-3949 2406-1026 1820-3949 |
DOI: | 10.2298/PIF1404249G |