Ageing-induced changes of reduced and oxidized glutathione in fragments of maize seedlings

• Published in: Journal of the Serbian Chemical Society Vol. 68; no. 12; pp. 911 - 918
• Main Authors: Dragicevic, Vesna, Sredojevic, Slobodanka, Spasic, Mihajlo, Vrvic, Miroslav
• Format: Journal Article
• Language: English
• Published: Serbian Chemical Society 2003
• Subjects: ageing; maize seedling; reduced and oxidised gluthatione
• ISSN: 0352-5139; 1820-7421
• DOI: 10.2298/JSC0312911D

A trial with four maize inbred lines with the ability to have different durations of seed germination in the course of the accelerated ageing (AA) treatment was set up. Changes of the content of total, reduced and oxidized glutathione (expressed as monomers) were observed in the seeds and seedlings before and after the treatment. For the first time, changes of glutathione in whole seedlings, as well as in the rest of the seed, were analyzed. It was noticed that maize in breds with a smaller decrease of the total glutathione but with an increase of the oxidized form had the ability of prolonged germination. In the control seed- lings, the amount of total glutathione was lower than in the treated ones. Maize seeds which lost germination faster had greater losses of total glutathione with an increased content of the oxidized form in seedlings. The ability of prolonged germination together with the possibility of glutathione synthesis in seedlings are genotypic traits. Postavljen je ogled sa cetiri linije kukuruza razlicite duzine ocuvanja klijavosti semena tokom tretmana ubrznog starenja. Ispitivane su promene ukupnog, kao i redukovanog i oksidovanog glutationa (izrazeni kao monomeri) u semenu i klijancima pre i nakon ubrzanog starenja. U istrazivanjima su prvi put analizirane promene glutationa u celim klijancima, kao i ostatku semena. U semenu linija kukuruza koje imaju sposobnost duzeg ocuvanja klijavosti bio je manji gubitak ukupnog glutationa, uz povecanje udela oksidovanog oblika. Kod njihovih klijanaca doslo je do povecanja sadrzaja ukupnog glutationa u odnosu na kontrolne klijance. Seme kukuruza koje brze gubi klijavost imalo je vece gubitke ukupnog glutationa, uz veci sadrzaj oksidovanog oblika kod formiranih klijanaca. Duzina ocuvanja klijavosti, kao i sinteza glutationa kod klijanaca je genotipska osobina.