Establishment of 43-plex SNP Typing System and Its Forensic Application

• Published in: Fa yi xue za zhi Vol. 34; no. 2; pp. 126 - 131,137
• Main Authors: Li, Y N, Li, M, Jiang, L, Luan, X H, Liang, N, Xu, Q N, Zhang, J S, Tang, M C, Bian, Y N, Chen, L Q
• Format: Journal Article
• Language: Chinese
• Published: China Editorial Office of Journal of Forensic Medicine 01.04.2018
• Subjects: Asian Continental Ancestry Group - genetics; China; Forensic Genetics; forensic genetics; polymorphism, genetic; matrix -assisted laser desorption -ionization; mass spectrometry; single nucleotide polymorphism; han nationality; eastern china; Gene Frequency; Genetic Markers; Genetics, Population; Genotype; Humans; Paternity; Polymorphism, Genetic; single nucleotide polymorphism; forensic genetics; Eastern China; Han nationality; mass spectrometry; polymorphism, genetic; matrix-assisted laser desorption-ionization
• ISSN: 1004-5619
• DOI: 10.3969/j.issn.1004-5619.2018.02.004

To evaluate the application of 43-plex SNP typing system in forensic science. The typing of 43 SNP loci in 123 unrelated Han individuals from East China was detected by MALDI-TOF-MS. The application value of 43-plex SNP typing system was assessed according to the forensic parameters of population genetics. All the 43 SNP loci of 123 individuals showed no significant departure from Hardy-Weinberg equilibrium （ >0.05）. Excepted rs1355366, rs2270529, rs10776839 and rs938283, there were 39 SNP loci had minor allele frequencies （MAF）, which were greater than 0.25. Among the 25 loci MAFs, 24 ranged from 0.4 to 0.5, while 3 were close to 0.4. The DP, CDP, PIC, Ho, PE and PE of the 43 SNP loci were 0.290 1-0.654 4, 1-9.8×10⁻¹¹, 0.170 8-0.500 0, 0.155 7-0.593 5, 0.085 4-0.250 0 and 0.014 6-0.125 0, respectively. The CPE and CPE were 0.999 986 and 0.992 436 1, respectively. The 43-plex SNP typing system in present study shows a high polymorphism, which can be an effective supplement and verification for traditional STR genetic markers. It also can be used with other commercial kits for the forensic paternity testing and individual identification.