Effect of tegoprazan, a novel potassium-competitive acid blocker, on non-steroidal anti-inflammatory drug (NSAID)-induced enteropathy

• Published in: Scientific reports Vol. 14; no. 1; pp. 27173 - 10
• Main Authors: Lee, Hyun Jung, Moon, Ji Wook, Koh, Seong-Joon, Im, Jong Pil, Kim, Byeong Gwan, Kim, Joo Sung
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 08.11.2024; Nature Publishing Group; Nature Portfolio
• Subjects: 631/250; 692/4020; Anti-inflammatory agents; Apoptosis; Cell proliferation; Cell viability; Cytokines; Enteropathy; Epithelial cells; Humanities and Social Sciences; Immunofluorescence; Indomethacin; Inflammation; multidisciplinary; Non-steroidal anti-inflammatory drug; Nonsteroidal anti-inflammatory drugs; Permeability; Science; Science (multidisciplinary); Small intestine; Tegoprazan; Western blotting; Zonula occludens-1 protein; Enteropathy; Tegoprazan; Permeability; Non-steroidal anti-inflammatory drug
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-024-78581-2

As the non-steroidal anti-inflammatory drugs (NSAIDs) are typically used in the treatment of chronic conditions, the incidence of NSAID-induced enteropathy is increasing. Given the challenges associated with discontinuing NSAIDs, effective preventive and treatment strategies are crucial. We assessed the effect of tegoprazan on NSAID-induced enteropathy. Human epithelial cells (HIEC-6, HT-29, and Caco-2) were treated with indomethacin and tegoprazan. Cell viability, expression levels of tight-junction proteins, levels of proinflammatory cytokines, and apoptosis were assessed by conducting MTT assays, RT-PCR, western blotting, and immunofluorescence staining, respectively. Tegoprazan significantly ameliorated the inhibition of cell proliferation induced by indomethacin. Tegoprazan also mitigated the suppression of occludin and ZO-1 expression by indomethacin, thereby restoring intestinal permeability. Additionally, tegoprazan reversed the indomethacin-induced elevation of the levels of proinflammatory cytokines and the rate of apoptosis of small intestinal epithelial cells. Our findings indicate that tegoprazan exerts a protective effect against NSAID-induced injury to small intestinal epithelial cells. The effect involves enhancement of the expression levels of tight junction proteins and the suppression of inflammation and apoptosis.