Cultivation model and deficit irrigation strategy for reducing leakage of bundle sheath cells to CO2, improve 13C carbon isotope, photosynthesis and soybean yield in semi-arid areas

• Published in: Journal of plant physiology Vol. 285; p. 153979
• Main Authors: Ren, Honglei, Zhang, Fengyi, Zhu, Xiao, Lamlom, Sobhi F., Liu, Xiulin, Wang, Xueyang, Zhao, Kezhen, Wang, Jinsheng, Sun, Mingming, Yuan, Ming, Gao, Yuan, Wang, Jiajun, Zhang, Bixian
• Format: Journal Article
• Language: English
• Published: Elsevier GmbH 01.06.2023
• Subjects: 13C carbon isotope; Cell leakage of bundle-sheath; Cultivation models; Irrigation strategies; Photosynthetic regulation; Soybean production; Cell leakage of bundle-sheath; 13C carbon isotope; Photosynthetic regulation; Irrigation strategies; Soybean production; Cultivation models
• ISSN: 0176-1617; 1618-1328
• DOI: 10.1016/j.jplph.2023.153979

A better understanding of the photosynthesis and soil water storage regulation of soybean production will be helpful to develop a water conservation strategy under a rain-fed farming system. Reducing the leakage of CO2 bundle sheath cells and improving the photosynthesis capacity and gas exchange characteristics of soybean leaves will contribute to increase yield under the dryland agricultural system and provide a scientific basis. Therefore, during 2019 and 2020, soybean exposed to different cultivation modes to analyze the response curves of photosynthesis and CO2 under different deficit irrigation strategies. In this study, we used two cultivation models: RB: ridge covered with biodegradable film and furrow area not covered; CF: conventional flat land planting under four deficit irrigation modes (R: rainwater irrigation; IB: branch stage irrigation (220 mm); IP: Irrigation during podding (220 mm); IBP: branch stage irrigation (110 mm), podding stage irrigation (110 mm). Compared with CF-IBP treatment, RB-IBP had significant effects on rainwater collection, SWS, and soybean yield. Photo-response curve analysis showed that RB-IBP treatment a significant increase in Pn, Gs, Ci, Tr, leaf WUE, and chlorophyll ab content. Under different irrigation strategies, maximum net photosynthetic rate (Pnmax), light saturation point (LSP), and apparent quantum efficiency under RB-IBP treatment (α), Pn under respiration rate and CO2 response curve were significantly higher than that under CF cultivation mode. Compared with RB culture mode under different irrigation strategies, CF cultivation mode significantly increases Δ13C and CO2 sheath cell leakage (Փ); it also led to a significant decline in the ratio of Ci/Ca concentration. This study shows that RB-IBP treatment is the best water-saving strategy because it means reducing the leakage of CO2 from the bundle sheath, thus significantly increasing soil water storage, photosynthetic capacity, and soybean yield.