Genomic Characterization of Antibiotic-Resistant Staphylococcus epidermidis with Observed Shifts in Optimal Temperature

• Published in: Journal of applied microbiology Vol. 135; no. 10
• Main Authors: Boyd, Sada M, Chacon-Barahona, Jonathan A, Mira, Portia, Dey, Debayan, Chun, Devyn, Xue, Carolyn, Pulido, Sophia, Yeh, Pamela
• Format: Journal Article
• Language: English
• Published: England 03.10.2024
• Subjects: microbial-cell interaction; stress response genes; antibiotic resistance; staphylococcus
• ISSN: 1365-2672; 1365-2672
• DOI: 10.1093/jambio/lxae252

Antibiotic resistance genes (ARGs) in the environment pose significant public health concerns and are influenced by conditions like temperature changes. We previously observed that resistance evolution to gentamicin and colistin affects optimal growth temperatures in S. epidermidis isolates. Despite significant phenotype observations, the genetic basis remains unclear. We aim to identify the genetic changes linked to antibiotic resistance evolution that alter optimal growth temperature. Using whole-genome sequencing, we sequenced the genomes of gentamicin-resistant (GEN-1, GEN-2) and colistin-resistant (COL-4, COL-6) S. epidermidis isolates. Variant analysis with the BV-BRC bioinformatics tool identified genes involved in antibiotic resistance and temperature response. We found 12 genetic variants, including two unique to GEN-2 and one in COL-4. One shared mutation was observed in GEN-1 and GEN-2, and another in COL-4 and COL-6. Five mutations were shared among all isolates related to mobile gene elements, including a transposase IS4 family, two putative transposases, and two transposase-like insertion elements. Our findings indicate that the same genes involved in gentamicin and colistin resistance, especially those related to mobile genetic elements, may also play a crucial role in temperature response.