Exploring native arsenic (As)-resistant bacteria: unveiling multifaceted mechanisms for plant growth promotion under As stress

• Published in: Journal of applied microbiology Vol. 135; no. 9
• Main Authors: Yan, Yaoyao, Chang, Wenying, Tian, Peili, Chen, Jiying, Jiang, Jiayin, Dai, Xianzhu, Jiang, Tao, Luo, Feng, Yang, Caiyun
• Format: Journal Article
• Language: English
• Published: England 02.09.2024
• Subjects: Arabidopsis - drug effects; Arabidopsis - growth & development; Arabidopsis - microbiology; Arsenic - metabolism; Brassica napus - growth & development; Brassica napus - microbiology; Chlorophyll - metabolism; Drug Resistance, Bacterial; Flavobacterium - drug effects; Flavobacterium - growth & development; Gibberellins - metabolism; Gibberellins - pharmacology; Lactuca - growth & development; Lactuca - microbiology; Oxidative Stress; Plant Development - drug effects; Plant Roots - growth & development; Plant Roots - microbiology; Seedlings - growth & development; Seedlings - microbiology; Siderophores - metabolism; Soil Microbiology; Stress, Physiological; Arabidopsis thaliana; plant growth promoting bacteria; arsenic resistant bacteria; arsenic; extracellular metabolites
• ISSN: 1365-2672; 1365-2672
• DOI: 10.1093/jambio/lxae228

This study explores the plant growth-promoting effect (PGPE) and potential mechanisms of the arsenic (As)-resistant bacterium Flavobacterium sp. A9 (A9 hereafter). The influences of A9 on the growth of Arabidopsis thaliana, lettuce, and Brassica napus under As(V) stress were investigated. Additionally, a metabolome analysis was conducted to unravel the underlying mechanisms that facilitate PGPE. Results revealed that A9 significantly enhanced the fresh weight of Arabidopsis seedlings by 62.6%-135.4% under As(V) stress. A9 significantly increased root length (19.4%), phosphorus (25.28%), chlorophyll content (59%), pod number (24.42%), and weight (18.88%), while decreasing As content (48.33%, P ≤ .05) and oxidative stress of Arabidopsis. It also significantly promoted the growth of lettuce and B. napus under As(V) stress. A9 demonstrated the capability to produce ≥31 beneficial substances contributing to plant growth promotion (e.g. gibberellic acid), stress tolerance (e.g. thiamine), and reduced As accumulation (e.g. siderophores). A9 significantly promoted the plant growth under As stress and decreased As accumulation by decreasing oxidative stress and releasing beneficial compounds.