Corning® Synthemax™ Surface: a tool for feeder-free, xeno-free culture of human embryonic stem cells

Currently, human embryonic stem cells (hESCs) are cultured on feeder cells or complex mixtures of proteins extracted from mouse tumors 1 , 2 . To allow commercialization of hESC-derived therapeutic cells, culture methods are required that are robust and scalable and that use chemically defined, xeno...

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Published inNature methods Vol. 7; no. 12; pp. 6 - 7
Main Authors Weber, Jennifer L, Dolley-Sonneville, Paula, Weber, David M, Fadeev, Andrei G, Zhou, Yue, Yang, Jiwei, Priest, Catherine A, Brandenberger, Ralph, Melkoumian, Zara
Format Journal Article
LanguageEnglish
Published New York Nature Publishing Group US 01.12.2010
Nature Publishing Group
Subjects
631/136/1660
631/136/532/2064/2117
631/1647/1407/651
application-note
Bioinformatics
Biological Microscopy
Biological Techniques
Biomedical Engineering/Biotechnology
Cell culture
Cell differentiation
Cell self-renewal
Commercialization
Culture
Differentiation (biology)
Embryo cells
Life Sciences
Proteomics
Stem cells
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Summary:Currently, human embryonic stem cells (hESCs) are cultured on feeder cells or complex mixtures of proteins extracted from mouse tumors 1 , 2 . To allow commercialization of hESC-derived therapeutic cells, culture methods are required that are robust and scalable and that use chemically defined, xeno-free materials. Corning Synthemax Surface is a novel synthetic surface that permits consistent long-term self-renewal of multiple hESC lines in defined, xeno-free media and differentiation of cells to functional cardiomyocytes 3 .
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ISSN:1548-7091
1548-7105
DOI:10.1038/nmeth.f.316