A benzothiazole-based fluorescent probe for distinguishing and bioimaging of Hg 2+ and Cu 2

A new benzothiazole-based fluorescent probe 2-(benzo[d]thiazol-2-yl)-4-(1,3- dithian-2-yl)phenol (BT) with two different reaction sites, a thioacetal group (site 1 for Hg ), and O and N atoms of the benzothiazole dye (site 2 for Cu ), was designed and synthesized. The probe BT showed ratiometric flu...

Full description

Saved in:
Bibliographic Details
Published inAnalytica chimica acta Vol. 954; pp. 97 - 104
Main Authors Gu, Biao, Huang, Liyan, Su, Wei, Duan, Xiaoli, Li, Haitao, Yao, Shouzhuo
Format Journal Article
LanguageEnglish
Published Netherlands 01.02.2017
Subjects
Benzothiazoles - chemistry
Copper - analysis
Fluorescent Dyes - chemistry
HeLa Cells
Humans
Ions - analysis
Mercury - analysis
Spectrometry, Fluorescence
Hg(2+) and Cu(2+)
Selectivity
Fluorescent probe
Fluorescent imaging
Online AccessGet full text

Cover

More Information
Summary:A new benzothiazole-based fluorescent probe 2-(benzo[d]thiazol-2-yl)-4-(1,3- dithian-2-yl)phenol (BT) with two different reaction sites, a thioacetal group (site 1 for Hg ), and O and N atoms of the benzothiazole dye (site 2 for Cu ), was designed and synthesized. The probe BT showed ratiometric fluorescent response to Hg and fluorescence quenching behavior to Cu , which induces naked-eye fluorescent color changes from green to blue and colorless, respectively. Moreover, it displayed highly sensitivity and selectivity toward Hg and Cu without interference from other metal ions. The sensing mechanisms were also confirmed by H NMR titration, mass spectrum and Job's plot analyses. Finally, probe BT was successfully used for fluorescent imaging of Hg and Cu in living cells, demonstrating its potential applications in biological science.
ISSN:0003-2670
1873-4324
DOI:10.1016/j.aca.2016.11.044