Assessment of genetic diversity of Stephania rotunda Lour. collected in Northern Vietnam using RAPD and ISSR markers

• Published in: Australian Journal of Crop Science Vol. 18; no. 7; pp. 374 - S6
• Main Authors: Ninh, Thi Thao, Nguyen, Tien Phat, Dang, Huyen Trang, Chu, Ha Duc, Dinh, Truong Son, Nguyen, Xuan Canh, Pham, Thi Dung, Nguyen, Thanh Hai, Nong, Thi Hue
• Format: Journal Article
• Language: English
• Published: Lismore, N.S.W Southern Cross Publishers 19.07.2024
• Subjects: Cluster analysis; Data processing; Genetics; Molecular aspects; Odor; Philosophy; Plants; Social aspects; Species
• ISSN: 1835-2693; 1835-2707
• DOI: 10.21475/ajcs.24.18.07.pne19

Stephania rotunda Lour. is a valuable traditional medicinal plant that is in danger of extinction. This research aimed to examine the genetic relationship of 32 Stephania rotunda Lour. accessions collected in Vietnam by using RAPD and ISSR markers. 14 RAPD and 14 ISSR primers successfully detected 163 loci of which 133 (80.19%) were polymorphic and a total of 3047 scorable bands were obtained. The 14 RAPD markers produced 1346 scorable bands, 89.63% of which were polymorphic while 14 ISSR primers resulted in 1701 bands, 70.76% of which were polymorphic. The RAPD primers showed a mean PIC of 0.3 and Rp value of 2.28 while the average PIC and Rp indexes for ISSR markers were 0.20 and 1.47, respectively. UPGMA dendrogram obtained from cluster analysis of RAPD and ISSR combined data grouped 32 accessions into five clusters at 76% variation with Jaccard's similarity coefficient varying from 0.586 to 0.951. Moreover, principal component analysis was also used to determine genetic relationships among 32 collected accessions. The results showed high genetic dissimilarity among 32 Stephania rotunda Lour. Correlation analysis between the matrices of similarity coefficient was measured using Mantel's test. A moderate correlation value (r equivalent 0.543) between RAPD vs ISSR matrices, but the strong correlation value between RAPD and pooled data (r equivalent 0.930) and between ISSR and combined matrices (r equivalent 0.814) were obtained, suggesting a combination of RAPD and ISSR markers appears to be productive in studying the genetic variance in Stephania rotunda Lour. Our results could provide a solid foundation for further conservation management and breeding programs of Stephania rotunda Lour.