Effect of Extracellular Vesicles Derived From Peripheral Blood Mononuclear Cells on K562 Leukemia Cell Line

Recently, extracellular vesicles (EVs) are generating considerable interest in terms of their ability. EVs defined themselves as a route for intercellular communication between the origin cells and the recipient ones by transferring information. This paper investigates the influence of peripheral bl...

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Bibliographic Details
Published inActa medica Iranica Vol. 61; no. 8
Main Authors Valandani, Hajar Mardani, Khalilabadi, Roohollah Mirzaee, Zahedi, Amir Mohammad
Format Journal Article
LanguageEnglish
Published Tehran University of Medical Sciences 18.02.2024
Subjects
Chronic myelogeneous leukaemia
Extracellular vesicles
k562 cell
Peripheral blood mononuclear cells
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Summary:Recently, extracellular vesicles (EVs) are generating considerable interest in terms of their ability. EVs defined themselves as a route for intercellular communication between the origin cells and the recipient ones by transferring information. This paper investigates the influence of peripheral blood mononuclear cells derived EVs on proliferation and apoptosis of the chronic myelogenous leukemia cell line k562. Trypan blue staining was used to detect cell viability subsequently, metabolic activity was assessed by the MTT assay. Cell apoptosis and cell cycle progression were evaluated using flowcytometric assay after treatment of k562 cell line with MNC derived EVs. Our results showed that MNC-EVs have no inhibitory effect on k562 cell growth and proliferation. Our data did not reveal any significant variations in the case of enhancing k562 cell line growth following treatment with MNC derived EVs. It seems tumour-derived extracellular vesicles support tumour cells growth by communicating with each other through their extracellular vesicles.
ISSN:0044-6025
1735-9694
DOI:10.18502/acta.v61i8.14901