A preliminary study on micronucleus analysis and nuclear anomalies in Pelophylax ridibundus (Pallas, 1771) (Amphibia: Anura) specimens collected around Vize (Kirklareli) and Ida Mountains (Canakkale, Turkey)

• Published in: Su ürünleri dergisi Vol. 29; no. 3; pp. 133 - 136
• Main Authors: Gurkan, M, Hayretdag, S, Yakyn, B Y, Tok, C V
• Format: Journal Article
• Language: English
• Published: 01.07.2013
• Subjects: Freshwater; Pelophylax ridibundus; Turkey, Inland Waters; Turkey, Marmara Region, Canakkale, Ida Mountains; Turkey, Marmara Region, Kirklareli, Vize
• ISSN: 1300-1590; 2148-3140
• DOI: 10.12714/egejfas.2012.29.3.05

In this study, a micronucleus analysis was made on the erythrocytes of the Pelophylax ridibundus specimens collected in Turkey. Nine (5 males and 4 females) P. ridibundus specimens were collected from Vize (Kirklareli) in April 2011 and five (3 males and 2 females) P. ridibundus specimens were collected from Ida Mountains (Yenice, Canakkale) in May 2011. The mean number of micronuclei was calculated as 6 plus or minus 4.17 and frequency as 0.3% in the specimens collected from Vize, while the mean number of micronuclei was calculated as 0.6 plus or minus 0.43 and frequency as 0.03% in the specimens collected from Ida Mountains. As a result of the statistical analyses, it was determined that there was a significant difference in the total number of micronuclei between both localities (p less than or equal to 0.05). Some 4 types of nuclear anomalies, i.e. binucleate, notched, blebbed and small-lobed, were detected in the study. No difference in the total number of nuclear anomalies was detected between the specimens of Vize and Ida Mountains (p=0.31). According to the results of the investigation, Vize samples have a higher frequency of micronuclei associated with intensive agricultural activities used genotoxic exposure to pesticides.