Biotin Regulation of Pancreatic Glucokinase and Insulin in Primary Cultured Rat Islets and in Biotin- Deficient Rats1
Biotin has been reported to affect glucose homeostasis; however, its role on pancreatic islets of Langerhans has not been assessed. In this report, we demonstrate that physiologic concentrations of biotin stimulate glucokinase activity in rat islets in culture. Using the branched DNA (bDNA) assay, a...
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Published in | Endocrinology (Philadelphia) Vol. 140; no. 10; pp. 4595 - 4600 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Endocrine Society
01.10.1999
|
Online Access | Get full text |
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Summary: | Biotin has been reported to affect glucose homeostasis; however, its
role on pancreatic islets of Langerhans has not been assessed. In this
report, we demonstrate that physiologic concentrations of biotin
stimulate glucokinase activity in rat islets in culture. Using the
branched DNA (bDNA) assay, a sensitive signal amplification technique,
we detected relative increases in glucokinase mRNA levels of 41.5±
13.% and 81.3 ± 19% at 12 and 24 h respectively in
islets treated with [10−6 m] biotin. Because
glucokinase activity controls insulin secretion, we also investigated
the effect of biotin on insulin release. Treatment with[
10−6 m] biotin for 24 h increased
insulin secretion. We extended our studies by analyzing the effect of
biotin deficiency on pancreatic islet glucokinase expression and
activity, as well as insulin secretion. Our results show that islet
glucokinase activity and mRNA are reduced by 50% in the biotin
deficient rat. Insulin secretion in response to glucose was also
impaired in islets isolated from the deficient rat. These data show
that biotin affects pancreatic islet glucokinase activity and
expression and insulin secretion in cultured islets. |
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ISSN: | 0013-7227 1945-7170 |
DOI: | 10.1210/endo.140.10.7084 |