Studies of the p53 gene mutation in Saudi non-Hodgkin's lymphoma

• Published in: Cancer letters Vol. 104; no. 2; pp. 225 - 232
• Main Authors: AHMAD, M. F, NASRIN, N, AKHTAR, M, HANNAN, M. A
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier 12.07.1996
• Subjects: Adolescent; Adult; Aged; Aged, 80 and over; Base Sequence; Biological and medical sciences; Child; Child, Preschool; DNA, Neoplasm - analysis; Exons; Female; Genes, p53; Hematologic and hematopoietic diseases; Humans; Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis; Lymphoma, Non-Hodgkin - genetics; Male; Medical sciences; Middle Aged; Molecular Sequence Data; Mutation; Polymorphism, Single-Stranded Conformational; Saudi Arabia; Asia; Human; Genetic inheritance; Lymphoproliferative syndrome; Etiopathogenesis; Malignant hemopathy; Mutation; Non Hodgkin lymphoma; Tumor suppressor gene; p53 gene
• ISSN: 0304-3835; 1872-7980
• DOI: 10.1016/0304-3835(96)04255-3

Tumor biopsies (paraffin embedded tissue) obtained from 45 Saudi patients with non-Hodgkin's lymphoma (NHL) were examined for the incidence of p53 mutations screened by polymerase chain reaction (PCR) and single strand conformation polymorphism (SSCP) analysis. DNA sequencing was carried out to confirm the occurrence of p53 mutation in PCR products showing abnormal migration by SSCP analysis. Only 1/45 samples showed the incidence of a homozygous mutation at codon 179 (exon 5) of the p53 gene that replaces histidine with tyrosine. The data showed that the frequency of p53 mutations was very low in Saudi NHL. Our results are consistent with the general observation that the p53 mutation is rather infrequent in hematopoietic malignancies like NHLs.