Advances in Studying Glycosaminoglycan-Protein Interactions Using Capillary Electrophoresis

Methods for studying interactions between glycosaminoglycans (GAGs) and proteins have assumed considerable significance as their biological importance increases. Capillary electrophoresis (CE) is a powerful method to study these interactions due to its speed, high efficiency, and low sample/reagent...

Full description

Saved in:
Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 2303; p. 365
Main Authors Liang, Aiye, Desai, Umesh
Format Journal Article
LanguageEnglish
Published United States 2022
Subjects
Electrophoresis, Capillary
Glycosaminoglycans
Proteins
Biophysical technique
GAG–protein interactions
Glycosaminoglycans
Capillary zone electrophoresis
Capillary electrophoresis
Affinity capillary electrophoresis
Heparin
Online AccessGet more information

Cover

More Information
Summary:Methods for studying interactions between glycosaminoglycans (GAGs) and proteins have assumed considerable significance as their biological importance increases. Capillary electrophoresis (CE) is a powerful method to study these interactions due to its speed, high efficiency, and low sample/reagent consumption. In addition, CE works effectively under a wide range of physiologically relevant conditions. This chapter presents the state of the art on CE methods for studying GAG-protein interactions including affinity capillary electrophoresis (ACE), capillary zone electrophoresis (CZE), frontal analysis (FA)/frontal analysis continuous capillary electrophoresis (FACCE), and capillary electrokinetic chromatography (CEC) with detailed experimental protocols for ACE and CZE methods.
ISSN:1940-6029
DOI:10.1007/978-1-0716-1398-6_30