Using a Suppression Subtractive Library-Based Approach to Identify Non-Heading Chinese Cabbage Genes Up-Regulated in Early Response to Elicitor PB90

• Published in: Agricultural sciences in China Vol. 7; no. 3; pp. 303 - 313
• Main Authors: GAN, Yun-zhe, ZHANG, Zheng-guang, WANG, Yuan-chao, ZHENG, Xiao-bo
• Format: Journal Article
• Language: English
• Published: Key Lab of Monitoring and Management of Plant Disease and Insects,Ministry of Agriculture/College of Plant Protection,Nanjing Agricultural University,Nanjing 210095.P.R.China 01.03.2008
• Subjects: 卷心菜; 大白菜; 种植技术; 识别模式; hypersensitive response; non-heading Chinese cabbage; up-regulated; PB90; suppression subtractive hybridization
• ISSN: 1671-2927
• DOI: 10.1016/S1671-2927(08)60070-X

Monitoring expression at the transcriptional level is the first essential step for the functional analysis of plant genes. Genes-encoding proteins directly involved in early response to elicitor constitute only a small fraction of all the genes affected by elicitor. Transcriptional responses to various elicitors have been extensively studied in different plants including Nicotiana and Arabidopsis thaliana; however, corresponding data aren＇t available for non-heading Chinese cabbage. To address this problem, we describe a suppression subtractive library-based approach to isolate the plant＇s ESTs up-regulated in the early induction/execution of the HR induced by elicitor PB90 from Phytophthora boehmeriae. According to their putative identification in BLAST searches against the three genome databases, 70 up-regulated genes were classified into 9 parts： some aspect of primary ＇metabolism＇ or ＇energy＇ production; ＇protein synthesis＇ or ＇protein fate＇; cellular communication/signal transduction mechanism; cell fates including Beclin, SPT1, and SPT2; HLA-B and AGO1 which participate in transcription; cellular transport and hypothetical proteins or proteins for which a function has yet to be determined. Seven selected genes such as Beclin, thioredoxin, HLA-B, MAP3K, SPT1, SPT2, and AGO1 were up-regulated induced by PB90, suggesting that the genes may play an important role in PB90-triggered HR.