Analysis of Differential Gene Expression Pattern in Brassica napus Hybrid Huayouza 6 and Its Parents Using Arabidopsis cDNA Microarray

• Published in: Agricultural sciences in China Vol. 5; no. 1; pp. 33 - 38
• Main Authors: SHEN, Jun-ru, WU, Jian-yong, ZHANG, Jian, LIU, Ping-wu, YANG, Guang-sheng
• Format: Journal Article
• Language: English
• Published: Yunnan Tobacco Research Institute, Yuxi 653100, P.R. China 2006; College of Plant Science & Technology, National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University,Wuhan 430070, P.R. China; China Tobacco Breeding Research (Southern) Center,Yuxi 653100,P.R.China%College of Plant Science & Technology, National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University,Wuhan 430070, P.R. China
• Subjects: 农业生物工程; 农作物; 多样性; 杂种优势; Brassica napus; heterosis; Arabidopsis cDNA-based microarray; Huayouza 6
• ISSN: 1671-2927
• DOI: 10.1016/S1671-2927(06)60016-3

Huayouza 6, a new semi-winter Brassica napus variety with high-yield, good quality, prematufity and extensive adaptability, was derived from the cross between the female parent 8086A and male parent 7-5. Two cDNA-based Arabidopisis microarray were used to analyze gene differential expression in bud of an elite B.napus hybrid Huayouza6 and its parents, in which there were 83 over-expression transcripts and 331 under-expression transcripts between Huayouza 6 and its female parent 8086A and 94 over-expression transcripts, and 423 under-expression transcripts were demonstrated between Huayouza 6 and its male parent 7-5. Further analysis showed that there were significant number of genes responsible for photosynthesis, and its implication for heterosis was discussed. Northern analysis of phosphoribulokinase coincided with its expression pattern derived from hybridization of Arabidopsis cDNA microarray and B. napus mRNA, this system of heterologous hybridization analysis should be applicable to other close relatives of Arabidopsis thaliana.