The Study of Selenium Fertilizer on the Growth of Xiangzayou 787 and Related Molecular Mechanism

• Published in: Agronomy (Basel) Vol. 14; no. 9; p. 2032
• Main Authors: Zhang, Qi, Peng, Jiayuan, Liu, Yuqi, Xie, Chunfeng, Zhang, Zhenqian
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 06.09.2024
• Subjects: Agricultural production; Bioinformatics; Budding; Chlorophyll; Correlation coefficient; Correlation coefficients; Edible oils; Fertilizers; Flowering; Gene expression; Gene sequencing; Leaves; Molecular modelling; Oilseeds; Phosphoprotein phosphatase; Physiology; Plant growth; Plant resistance; Protein phosphatase; protein phosphatase 2C gene; Rapeseed; Raw materials; Seedlings; Seeds; Selenium; selenium fertilizer; Sequence analysis; Silicon; soluble sugar; Sugar; Transcriptomes; Xiangzayou 787; China
• ISSN: 2073-4395; 2073-4395
• DOI: 10.3390/agronomy14092032

Rapeseed is the largest self-produced oil crop in China which plays an important role in ensuring the safety of edible oil. However, its current per unit yield is far below Canada and Europe. In this study, selenium fertilizer and other micro fertilizers were sprayed on Xiangzayou 787 at the seedling stage. The results showed that the yield per plant increased 24.3% with sprayed selenium compared to the control (CK). Compared with the CK, the chlorophyll content in leaves at the flowering stage was significantly increased by 20.8%, and the soluble sugar content in the silique wall and seeds at the maturity stage was significantly increased and increased by 62.1% during the budding stage. The functional leaves of Xiangzayou 787 with the sprayed selenium fertilizer and the CK were used as raw materials for absolute quantitative transcriptome sequencing analysis. Accompanied with bioinformatics analysis, six differential genes which affect growth were discovered. The expression level of the protein phosphatase 2C gene in the silique wall was significantly higher than that of the CK. The PP2C78 gene was significantly positively correlated with the chlorophyll and soluble sugar content in leaves and the correlation coefficients were 0.539 and 0.547. According to gene expression levels, yield, and physiological indicators, PP2C78 may be a key functional gene affecting rapeseed yield. In this study, selenium fertilizer was found to be an excellent foliar fertilizer for rapeseed; the PP2C78 gene may be helpful for analyzing the yield increasing mechanism and used as a reference for screening new foliar fertilizers.