Indirect Immunofluorescence of Tissue Sections

Immunofluorescence (IF) on tissue sections allows for the detection of protein species subcellular localization. IF studies further offer the ability to achieve this understanding at the level of single cell granularity. Here, we describe the processes by which tissue is fixed, embedded, sectioned,...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 2386; p. 17
Main Author Aros, Cody J
Format Journal Article
LanguageEnglish
Published United States 2022
Subjects
Fluorescent Antibody Technique, Indirect
Staining and Labeling
Tissue Fixation
Blocking
Permeabilization
Antigen retrieval
Immunofluorescence
Antibody
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Summary:Immunofluorescence (IF) on tissue sections allows for the detection of protein species subcellular localization. IF studies further offer the ability to achieve this understanding at the level of single cell granularity. Here, we describe the processes by which tissue is fixed, embedded, sectioned, and subsequently utilized for conducting indirect IF assays. We raise potential opportunities for troubleshooting and optimization at varying stages of the protocol.
ISSN:1940-6029
DOI:10.1007/978-1-0716-1771-7_2