Large-Scale Transient Production in ExpiCHO-S™ with Enhanced N-Galactosylation-Sialylation and PEI-Based Transfection

Large-scale transient expression in Chinese Hamster Ovary (CHO) cells provides a rapid protein production method with a potential start-to-end alignment advantage for biotherapeutics drug discovery. In this chapter, experimental protocols are illustrated for transient expression of therapeutic glyco...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 2313; p. 143
Main Authors Zhong, Xiaotian, Schwab, Ashley, Ma, Weijun, Meade, Caryl L, Zhou, Jing, D'Antona, Aaron M, Somers, Will, Lin, Laura
Format Journal Article
LanguageEnglish
Published United States 2022
Subjects
Animals
CHO Cells
Cricetinae
Cricetulus
Protein Processing, Post-Translational
Recombinant Proteins
Transfection
Polyethylenimine
Therapeutic glycoproteins
Transfection
Chinese Hamster Ovary (CHO) Cells
N-linked glycosylation and sialylation
Transient expression
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Summary:Large-scale transient expression in Chinese Hamster Ovary (CHO) cells provides a rapid protein production method with a potential start-to-end alignment advantage for biotherapeutics drug discovery. In this chapter, experimental protocols are illustrated for transient expression of therapeutic glycoproteins with improved galactosylation and sialylation in ExpiCHO-S™ system. To reduce the production cost, we also describe a novel procedure for PEI-mediated transfection in ExpiCHO-S™ cells that supports therapeutic protein expression comparable to the level with ExpiFectamine™-based transfection.
ISSN:1940-6029
DOI:10.1007/978-1-0716-1450-1_7