Chapter 7. Nonsense-mediated mRNA decay in Caenorhabditis elegans

The nonsense-mediated mRNA decay (NMD) pathway is a surveillance mechanism that targets the degradation of mRNAs harboring premature termination codons (PTCs). Two key aspects of NMD are the definition of a PTC codon and the identification of the molecular machinery dedicated to this mechanism. This...

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Bibliographic Details
Published inMethods in enzymology Vol. 449; p. 149
Main Authors Longman, Dasa, Arrisi, Pablo, Johnstone, Iain L, Cáceres, Javier F
Format Journal Article
LanguageEnglish
Published United States 2008
Subjects
Animals
Caenorhabditis elegans - genetics
Codon, Nonsense - genetics
RNA Interference - physiology
RNA Stability - genetics
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Summary:The nonsense-mediated mRNA decay (NMD) pathway is a surveillance mechanism that targets the degradation of mRNAs harboring premature termination codons (PTCs). Two key aspects of NMD are the definition of a PTC codon and the identification of the molecular machinery dedicated to this mechanism. This chapter describes the development of transgenic reporters as well as the use of genome-wide RNAi and genetic screens to identify novel components of the NMD pathway in the nematode Caenorhabditis elegans.
ISSN:1557-7988
DOI:10.1016/S0076-6879(08)02407-5