Study of Molecular Parameters and Antibacterial Activity of a Peptide Preparation Derived from Human Leukocytes

Background. The study of new antimicrobial substances isolated from the living organism tissues is of particular importance for medicine and the biopharmaceutical industry. Leukocyte peptides can act as an alternative to traditional antibiotics to overcome the bacterial antibiotic resistance problem...

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Published inАнтибиотики и Химиотерапия Vol. 68; no. 9-10; pp. 20 - 24
Main Authors A. G. Volkov, L. I. Kononova, V. P. Korobov, L. V. Volkova
Format Journal Article
LanguageRussian
Published LLC "Publishing House OKI" 15.01.2024
Subjects
antibacterial activity
human leukocyte peptides
reversed phase chromatography
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Summary:Background. The study of new antimicrobial substances isolated from the living organism tissues is of particular importance for medicine and the biopharmaceutical industry. Leukocyte peptides can act as an alternative to traditional antibiotics to overcome the bacterial antibiotic resistance problem.Aim. Evaluation of molecular weight and biological activity of ultrasound-derived leukocyte peptides.Methods. Separation of leukocyte peptide complexes into particular fractions by reverse phase chromatography and evaluation of their antibacterial activity by serial dilution and diffusion methods.Results. Studies have shown that one peptide fraction with a molecular weight of 440–570 Da has pronounced antibacterial activity.Conclusion. The obtained experimental data testify to the high therapeutic potential of the leukocyte protein-peptide complex, which has a wide spectrum of antibacterial activity.
ISSN:0235-2990
DOI:10.37489/0235-2990-2023-68-9-10-20-24