Cytokine-Activated Endothelium Recruits Osteoclast Precursors
Osteoclast precursors reach sites of osteoclast formation and remodelling via the vasculature and are therefore destined to encounter endothelium before migrating to the bone surface. Here we investigated the hypothesis that endothelium may be involved in the regulation of osteoclast precursor recru...
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Published in | Endocrinology (Philadelphia) Vol. 142; no. 4; p. 1678 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Endocrine Society
01.04.2001
|
Online Access | Get full text |
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Summary: | Osteoclast precursors reach sites of osteoclast formation and
remodelling via the vasculature and are therefore destined to encounter
endothelium before migrating to the bone surface. Here we investigated
the hypothesis that endothelium may be involved in the regulation of
osteoclast precursor recruitment to sites of bone resorption.
Osteoclast precursors in human peripheral blood were identified by
their ability to form mature osteoclasts in 21-day cultures
supplemented with RANKLigand, M-CSF, 1,25(OH)2-vitamin
D3, dexamethasone and prostaglandin E2.
Under control conditions few osteoclast precursors adhered to
endothelial cells (the human bone marrow-derived endothelial cell line
BMEC-1). However, BMEC-1 cells treated with the resorption stimulating
cytokines IL-1β and TNFα depleted the PBMC population of all
osteoclast precursors. These results provide the first evidence that
osteoclast precursors can adhere to endothelium and suggest that
endothelium could play an important role in the recruitment of
osteoclast precursors to sites of bone resorption. |
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ISSN: | 0013-7227 1945-7170 |
DOI: | 10.1210/endo.142.4.8204 |