Fluorescence In Situ Hybridization to Maize (Zea mays) Chromosomes

Fluorescence In Situ Hybridization (FISH) is the annealing of fluorescent DNA probes to their complementary sequences on prepared chromosomes and subsequent visualization with a fluorescent microscope. In maize, FISH is useful for distinguishing each of the ten chromosomes in different accessions (k...

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Bibliographic Details
Published inCurrent protocols in plant biology Vol. 1; no. 3; p. 530
Main Authors McCaw, Morgan, Graham, Nathaniel, Cody, Jon, Swyers, Nathan, Zhao, Changzeng, Birchler, James
Format Journal Article
LanguageEnglish
Published United States 01.09.2016
Subjects
fluorescence in situ hybridization
FISH
maize (Zea mays)
karyotyping
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Summary:Fluorescence In Situ Hybridization (FISH) is the annealing of fluorescent DNA probes to their complementary sequences on prepared chromosomes and subsequent visualization with a fluorescent microscope. In maize, FISH is useful for distinguishing each of the ten chromosomes in different accessions (karyotyping), roughly mapping single genes, transposable elements, transgene insertions, and identifying various chromosomal alterations. FISH can also be used to distinguish chromosomes between different Zea species in interspecific hybrids by use of retroelement painting. © 2016 by John Wiley & Sons, Inc.
ISSN:2379-8068
DOI:10.1002/cppb.20033