Micrococcus luteus Deoxyribonucleic Acid Polymerase

The Micrococcus luteus DNA polymerase was purified over 1200-fold. The enzyme replicates, with varying efficiency, a wide variety of naturally occurring DNA's, including double stranded, single stranded, circular, and denatured DNA's. Also, a variety of DNA polymers containing defined repe...

Full description

Saved in:
Bibliographic Details
Published inThe Journal of biological chemistry Vol. 245; no. 21; pp. 5614 - 5624
Main Authors Harwood, Steven J., Schendel, Paul F., Wells, Robert D.
Format Journal Article
LanguageEnglish
Published American Society for Biochemistry and Molecular Biology 10.11.1970
Online AccessGet full text

Cover

More Information
Summary:The Micrococcus luteus DNA polymerase was purified over 1200-fold. The enzyme replicates, with varying efficiency, a wide variety of naturally occurring DNA's, including double stranded, single stranded, circular, and denatured DNA's. Also, a variety of DNA polymers containing defined repeating nucleotide sequences are replicated; many-fold synthesis is customarily observed. Replication is completely accurate as judged by nearest neighbor frequency analyses and analytical ultracentrifugal studies on the product DNA. When native Bacillus subtilis transforming DNA is replicated, however, the product DNA is not genetically active and is renaturable. The polymerase efficiently and faithfully repairs DNA's that contain partially single stranded regions.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(18)62699-7