Effects of amixine on electrophoretic mobility of murine T lymphocytes

• Published in: Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994) Vol. 57; no. 2; pp. 14 - 18
• Main Authors: Dolha, O V, Pohoriela, N Kh, Bohorad-Kobel's'ka, O S, Andronati, S A, Mahura, I S
• Format: Journal Article
• Language: English; Ukrainian
• Published: Ukraine 2011
• Subjects: Animals; Cell Movement - drug effects; Cells, Cultured; Dose-Response Relationship, Drug; Electrophoresis; Interferon Inducers - pharmacology; Male; Membrane Potentials - drug effects; Mice; Mice, Inbred CBA; Spleen - cytology; Spleen - drug effects; Spleen - immunology; T-Lymphocytes - drug effects; T-Lymphocytes - immunology; Tilorone - pharmacology
• ISSN: 2522-9028; 2522-9036
• DOI: 10.15407/fz57.02.014

The amixine-induced early changes in the electrophoretic mobility (EPM) of murine splenic T lymphocytes were studied in vitro by the microelectrophoresis technique. It has been found that T lymphocytes treated with amixine have a greater EPM within the first hours of amixine addition than control cells. This change in EPM depends on the concentration of amixine in the medium and the duration ofamixine exposure. It was concluded that the amixine-treated cells have a greater net negative surface charge density than control cells. This effect may play an important role in the cell-cell interaction during the immune response.