Studies on the Biosynthesis of Mannan in Micrococcus lysodeikticus

The carrier lipid involved in the enzymatic synthesis of mannosyl-1-phosphoryl-undecaprenol was isolated from the crude lipids of Micrococcus lysodeikticus and identified as undecaprenyl phosphate. In the presence of a particulate enzyme from M. lysodeikticus undecaprenyl phosphate serves as an acce...

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Published inThe Journal of biological chemistry Vol. 244; no. 21; pp. 5890 - 5898
Main Authors Lahav, Meir, Chiu, T.H., Lennarz, W.J.
Format Journal Article
LanguageEnglish
Published American Society for Biochemistry and Molecular Biology 10.11.1969
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Summary:The carrier lipid involved in the enzymatic synthesis of mannosyl-1-phosphoryl-undecaprenol was isolated from the crude lipids of Micrococcus lysodeikticus and identified as undecaprenyl phosphate. In the presence of a particulate enzyme from M. lysodeikticus undecaprenyl phosphate serves as an acceptor of a mannosyl group from GDP-mannose, with concomitant production of GDP. Optimal rates of enzymatic synthesis of mannosyl-1-phosphoryl-undecaprenol require the presence of Mg ++ ion, and a surfaceactive compound, such as phosphatidylglycerol. An isomer of undecaprenyl phosphate, ficaprenyl (11) phosphate, serves as a substrate for mannosyl-1-phosphoryl-undecaprenol synthetase.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(18)63557-4