Investigation of Rapid Detection Method for Campylobacter jejuni/coli in Human Diarrheal Feces Using Three Different DNA-Based Methods

• Published in: Journal of the Japan Veterinary Medical Association Vol. 66; no. 7; pp. 483 - 487
• Main Author: ONO, Kazuaki
• Format: Journal Article
• Language: Japanese; English
• Published: Japan Veterinary Medical Association 20.07.2013
• Subjects: Campylobacter jejuni/coli; LAMP; patient feces; PCR; real-time PCR
• ISSN: 0446-6454; 2186-0211
• DOI: 10.12935/jvma.66.483

The results of Campylobacter jejuni/coli detection obtained using the three DNA-based methods PCR, loop-mediated isothermal amplification (LAMP) and real-time PCR were compared to those obtained by conventional culture. By culturing, C. jejuni/coli was detected in 42/55 (76.4%) samples, whereas 42/55 (76.4%) were found to be positive using PCR, 49/55 (89.1%) were found to be positive using LAMP, and 50/55 (90.9%), were found to be positive using real-time PCR. The DNA-based methods were more sensitive than culturing, with three (23.1%), seven (53.8%) and eight (61.5%) of 13 culture-negative samples found to be C. jejuni/coli positive using the PCR, LAMP and real-time PCR methods, respectively. Although there were several false negative cases observed, the 5-10-fold dilution of the samples with distilled water facilitated the positive reaction. The DNA-based methods were rapid and thought to be substantially more effective in detecting C. jejuni/coli in diarrheal feces than conventional culturing.