Antineutrophil cytoplasmic autoantibodies (ANCA) recognizing a recombinant myeloperoxidase subunit

Sera from 76 patients with diverse vasculitis, systemic lupus erythematosus (SLE) and hydralazine-induced lupus (HiL) were analysed by ELISA for their reactivity with native, reduced or urea-denatured MPO, respectively, as well as with bacterially expressed heavy and light subunits of MPO. All sera...

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Bibliographic Details
Published inAdvances in experimental medicine and biology Vol. 336; p. 87
Main Authors Pedrollo, E, Bleil, L, Bautz, F A, Kalden, J R, Bautz, E K
Format Journal Article
LanguageEnglish
Published United States 1993
Subjects
Antibodies, Antineutrophil Cytoplasmic
Antibody Specificity
Autoantibodies - immunology
Enzyme-Linked Immunosorbent Assay
Epitopes - immunology
Granulomatosis with Polyangiitis - immunology
Humans
Lupus Erythematosus, Systemic - immunology
Peroxidase - immunology
Protein Denaturation
Recombinant Proteins - immunology
Urea
Vasculitis - immunology
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Summary:Sera from 76 patients with diverse vasculitis, systemic lupus erythematosus (SLE) and hydralazine-induced lupus (HiL) were analysed by ELISA for their reactivity with native, reduced or urea-denatured MPO, respectively, as well as with bacterially expressed heavy and light subunits of MPO. All sera (n = 20) recognizing native MPO showed a positive reaction with reduced MPO, while 12 recognized the denatured protein. Most of the linear epitopes are located in the light subunit, since 9 MPO-positive sera recognized significantly the bacterially expressed, denatured light subunit, while only one serum recognized the bacterially expressed heavy subunit purified under denaturing conditions.
ISSN:0065-2598
DOI:10.1007/978-1-4757-9182-2_13