Diuretic efficacy and prophylactic effects of hydroethanolic extract from Musa balbisiana fruits against urolithiasis

• Published in: Advances in traditional medicine (Online) Vol. 22; no. 4; pp. 823 - 836
• Main Authors: Ly, Hai Trieu, Le, Thi Kim Oanh, Nguyen, Minh Khoi, Le, Van Minh
• Format: Journal Article
• Language: English
• Published: Singapore Springer Nature Singapore 01.12.2022
• Subjects: Biomedical and Life Sciences; Biomedicine; Pharmacy; Research Article; Biological activities; fruits; Urolithiasis; Diuretic effect
• ISSN: 2662-4052; 2662-4060
• DOI: 10.1007/s13596-022-00629-3

The use of herbs as prophylactic and prevent recurrence of urolithiasis was counseled. Musa balbisiana fruits have long been used folk in the treatment of urinary stones. However, there is no clear scientific evidence. This study was designed to investigate the diuretic and prophylactic effects of M. balbisiana fruit extract in ethylene glycol-induced urolithiasis in rats. In this study, crude extracts were extracted by maceration, hot extraction, and decoction methods. The extracts were examined in vitro biological activities of anti-urolithiatic, anti-inflammatory, antibacterial, and antioxidant properties. For in vivo study, the experimental groups of rats were given the extract for 7-day to evaluate the diuretic potential. In prophylactic activity against urolithiasis, rats were given the extract for 28-day on ethylene glycol-induced urolithiasis in male Wistar rats. The results showed that all extracts of M. balbisiana fruits exhibited in vitro anti-urolithiatic, anti-inflammatory, antibacterial, and antioxidant activities with the hydroethanolic extract by hot extraction presented the highest activities. The hydroethanolic extract at the doses of 0.8 and 1.6 g/kg had diuretic effect after 7-day of treatment. The extract at these two doses had significantly prophylactic activity restored the parameters in urine and serum to near-normal level. The histopathological examinations revealed that calcium oxalate crystal deposits in the renal tubules and congestion and dilation of the renal tubules were significantly reverted after the extract treatment for 28-day. Taken together, the hydroethanolic extract of M. balbisiana fruits had diuretic effect and reduced the growth of urinary stones showing its effect as an antiurolithiatic support agent.