Binding of Bilirubin with Lipid
Binding of bilirubin with mitochondria, mitochondrial membranes, various cells, and cellular fragments resulted in a hypochromicity with a red shift of the absorption spectrum of bilirubin. It was found that the mitochondrial lipids rather than the proteins were involved in the binding. Isolated mit...
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| Published in | The Journal of biological chemistry Vol. 245; no. 5; pp. 1084 - 1089 |
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| Main Authors | , |
| Format | Journal Article |
| Language | English |
| Published |
American Society for Biochemistry and Molecular Biology
10.03.1970
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| Online Access | Get full text |
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| Summary: | Binding of bilirubin with mitochondria, mitochondrial membranes, various cells, and cellular fragments resulted in a hypochromicity
with a red shift of the absorption spectrum of bilirubin. It was found that the mitochondrial lipids rather than the proteins
were involved in the binding. Isolated mitochondrial lipid and lipids from various other sources caused similar spectral changes
of the bilirubin absorbance. Exogenous lipid could prevent the toxic effect of bilirubin on well defined mitochondrial reactions
as did serum albumin.
Washing of bilirubin-treated mitochondria with albumin restored their normal function to a considerable extent. These findings
suggest that the binding of bilirubin with lipid alters the characteristics of mitochondrial membranes so that membrane-linked
functions are impaired. In view of the lipophilic nature of bilirubin, this explanation might be considered as a mechanism
of toxicity of bilirubin for cells in general. |
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| ISSN: | 0021-9258 1083-351X |
| DOI: | 10.1016/S0021-9258(18)63292-2 |