Isolation and characterization of Frankia sp. strain FaCl genes involved in nitrogen fixation

Genomic DNA was isolated from Frankia sp. strain FaCl, an Alnus root nodule endophyte, and used to construct a genomic library in the cosmid vector pHC79. The genomic library was screened by in situ colony hybridization to identify clones of Frankia nitrogenase (nif ) genes based on DNA sequence hom...

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Bibliographic Details
Published inApplied and environmental microbiology Vol. 53; no. 10; pp. 2321 - 2327
Main Authors Ligon, J M, Nakas, J P
Format Journal Article
LanguageEnglish
Published 01.10.1987
Subjects
DNA
Frankia
genes
nitrogen fixation
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Summary:Genomic DNA was isolated from Frankia sp. strain FaCl, an Alnus root nodule endophyte, and used to construct a genomic library in the cosmid vector pHC79. The genomic library was screened by in situ colony hybridization to identify clones of Frankia nitrogenase (nif ) genes based on DNA sequence homology to structural nitrogenase genes from Klebsiella pneumoniae . Several Frankia nif clones were isolated, and hybridization with individual structural nitrogenase gene fragments (nifH, nifD , and nifK ) from K. pneumoniae revealed that they all contain the nifD and nifK genes, but lack the nifH gene. Restriction endonuclease mapping was preformed.
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ISSN:0099-2240
DOI:10.1128/AEM.53.10.2321-2327.1987