The expression of microRNA-10a-5p and microRNA-34c-5p with target proteins in laryngeal epithelial premalignant lesions

• Published in: Pathology Vol. 46; p. S117
• Main Author: Liu, Honggang
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 2014
• ISSN: 0031-3025; 1465-3931
• DOI: 10.1097/01.PAT.0000454497.39850.36

There is still no general agreement among multidisciplinary fields for the histological classification, clinical management, and prognosis for laryngeal epithelial premalignant lesion (LEPL). Moreover, few molecular markers were found for these challenging lesions. In this study, we analyzed inter-observer histopathological variability and the carcinoma transformation of LEPL. The expression of miR-10a and miR-34c was identified in LEPL by qRT-PCR, and the correlation of miRs with clinicopathology of LEPL was analyzed by linear regression model. Then the target genes of miRs were predicted and vertified in LEPL in order to analyze the contribution of miRs with target genes in carcinogenesis of laryngeal carcinoma. There was a moderate agreement in classification of LEPL. The expression of miR-10a and miR-34c with their target genes (HOXA1, β-Catenin and Cortactin, CyclinD1) were dysregulated respectively in LEPL, which might provide an important role in diagnosis of LEPL to distinguish low-risk and high-risk lesions. The differential expression of miR-34c showed correlation with alcohol consumption, and the high expression of HOXA1 and CyclinD1 may have prognostic value in guiding LEPL, which may act as valuable molecular markers in targeted therapy for early laryngeal carcinoma.