tmRNA abundance inStreptomyces aureofaciens, S. griseus andS. collinus under stress-inducing conditions

• Published in: Folia microbiologica Vol. 52; no. 5; pp. 463 - 470
• Main Authors: Palečková, P., Felsberg, J., Bobek, J., Mikulík, K.
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Nature B.V 01.09.2007
• Subjects: Decay; Protein synthesis; Proteins; Sodium chloride; Streptomycetes
• ISSN: 0015-5632; 1874-9356
• DOI: 10.1007/BF02932105

tmRNA and protein SmpB are the main components required for rescue of stalled ribosomes incapable of properly elongating or terminating the polypeptide chain. We examined the tmRNA level and protein synthesis inStreptomyces aureofaciens, S. griseus andS. collinus synthesizing tetracycline, streptomycin and kirromycin, respectively, during various stress conditions. Downshift in temperature caused a decrease in protein synthesis but the level of tmRNA increased. Shift up in temperature induced decay of tmRNA in all strains and inS. collinus led to stimulation and inS. aureofaciens andS. griseus to inhibition of protein synthesis. At high NaCl concentrations protein synthesis was inhibited and tmRNA decayed. Shift in pH from 7.0 to 5.0 had no pronounced effect on the tmRNA level while upshift to pH 9.0 inS. collinus andS. aureofaciens caused inhibition of protein synthesis and decay of tmRNA inS. collinus. In contrast, protein synthesis and tmRNA level increased inS. griseus at the alkaline pH. Our data show that tmRNA abundance is important for survival of streptomycetes under certain unfavorable conditions.[PUBLICATION ABSTRACT]