Inhibition of trypsin-induced mast cell activation by water fraction ofLonicera japonica

• Published in: Archives of pharmacal research Vol. 27; no. 11; pp. 1141 - 1146
• Main Authors: Kang, Ok-Hwa, Choi, Yeon-A, Park, Hye-Jung, Lee, Joo-Young, Kim, Dae-Ki, Choi, Suck-Chei, Kim, Tae-Hyun, Nah, Yong-Ho, Yun, Ki-Jung, Choi, Suck-Jun, Kim, Young-Ho, Bae, Ki-Hwan, Lee, Young-Mi
• Format: Journal Article
• Language: English
• Published: Pharmaceutical Society of Korea 01.11.2004
• Subjects: dose response; enzyme-linked immunosorbent assay; gene expression; Lonicera japonica; mast cells; messenger RNA; mitogen-activated protein kinase; phosphorylation; reverse transcriptase polymerase chain reaction; secretion; trypsin; tryptase; tumor necrosis factor-alpha; Western blotting
• ISSN: 0253-6269; 1976-3786
• DOI: 10.1007/BF02975120

Lonicera japonica Thunb.(Caprifoliaceae) has long been known as an anti-inflammatory. In the present study, the effect of water fraction ofLonicera japonica (LJ) on trypsin-induced mast cell activation was examined. HMC-1 cells were stimulated with trypsin (100 nM) in the presence or absence of LJ (10, 100, and 1000 μg/mL). TNF-α and tryptase production were measured by enzyme-linked immunosorbent assay (ELISA) and reverse transcription-PCR. Extracellular signal-regulated kinase (ERK) phosphorylation was assessed by Western blot. Trypsin activity was measured by using Bz-DL-Arg-p-nitroanilide (BAPNA) as substrate. LJ (10, 100, and 1000 μg/mL) inhibited TNF-α secretion in a dose-dependent manner. LJ (10, 100, and 1000 μg/mL) also inhibited TNF-α and tryptase mRNA expression in trypsin-stimulated HMC-1. Furthermore, LJ inhibited trypsin-induced ERK phosphorylation. However, LJ did not affect the trypsin activity even 1000 μg/mL. These results indicate that LJ may inhibit trypsin-induced mast cell activation through the inhibition of ERK phosphorylation than the inhibition of trypsin activity.