Investigation of the effect of REM sleep deprivation on epileptic seizures caused by pentylenetetrazole in mice
Aim: To investigate whether different periods of rapid eye movement sleep deprivation (REM SD) contribute to seizure susceptibility, hippocampal oxidative status and balance of inhibition-excitation in the acute epilepsy model. Methods: REM SD was performed using the modified multiple platforms meth...
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Published in | Experimental biomedical research Vol. 3; no. 3; pp. 149 - 156 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Bolu
Prof. Dr. Hayrettin Öztürk
01.07.2020
Experimental Biomedical Research |
Subjects | |
Online Access | Get full text |
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Summary: | Aim: To investigate whether different periods of rapid eye movement sleep deprivation (REM SD)
contribute to seizure susceptibility, hippocampal oxidative status and balance of inhibition-excitation
in the acute epilepsy model.
Methods: REM SD was performed using the modified multiple platforms method on adult male
BALB/c mice. Pentylentetrazol (PTZ) was injected to induce seizures and hippocampal total
antioxidant status (TAS), total oxidant status (TOS), gamma aminobutyric acid (GABA), and
glutamate levels were measured using the ELISA method.
Results: PTZ-induced seizures following 8 h and 72 h REM SD significantly reduced the
hippocampal TAS levels, but did not affect the TOS levels. In REM SD groups, especially after 8
hours of REM sleep loss, there was a significant increase in glutamate in PTZ induction. The
hippocampal GABA levels were increased by PTZ-induced seizures after 72 h REM SD. PTZinduction
after 8 hours of RAM SD leads to a significant increase in the seizure duration.
Conclusion: It can be speculated that the REM SD can contribute to seizure susceptibility by
changing the oxidant-antioxidant balance and excitatory and inhibitory tone in the hippocampus. |
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ISSN: | 2618-6454 2618-6454 |
DOI: | 10.30714/j-ebr.2020361050 |