A comparison of15N proline and13C leucine for monitoring protein biosynthesis in the skin

• Published in: Amino acids Vol. 16; no. 2; pp. 107 - 111
• Main Authors: Doumit, J., Le, J., Frey, J., Chamson, A., Perier, C.
• Format: Journal Article
• Language: English
• Published: Vienna Springer Nature B.V 01.06.1999
• Subjects: Proteins
• ISSN: 0939-4451; 1438-2199
• DOI: 10.1007/BF01321530

The tracers L^sup 15^N-proline and L(1-^sup 13^C)-leucine were used to explore the synthesis of skin proteins in vivo in rabbits. They orally received a single dose containing an equimolecular mixture of L(1-^sup 13^C)-leucine and L^sup 15^N-proline. The changes in the amounts of these tracers in blood and skin were monitored for a total of 8 h. The data showed the appearance of the two tracers in blood within 15 min and their clearance in 8h. They were both rapidly (15 min) incorporated into skin proteins, but more proline was incorporated than leucine. We therefore consider L^sup 15^N-proline to be a better tracer than L(1-^sup 13^C)-leucine for studying protein metabolism in the skin.[PUBLICATION ABSTRACT]