Genetic polymorphism of 15 STR loci in Tibetan population

The polymorphism distributions of 15 STR loci (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, VWA, TPOX, D18S51, D5S818, and FGA) were investigated in a Tibetan population by multiplex PCR amplification using five fluorochromes (6FAM, VIC, NED, PET, LIZ). Gene fr...

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Published inYíchuán Vol. 30; no. 7; p. 851
Main Authors Li, Ning, Su, Yu-Hong, Xi, Huan-Jiu, Ren, Fu, Huang, Ke-Qiang, Xiao, Yan-Jie, Wen, You-Feng, Li, Chun-Shan, Li, Chang-Yong, Wang, Kun, Cheng, Jian-Guo
Format Journal Article
LanguageChinese
Published China 01.07.2008
Subjects
Gene Frequency - genetics
Genetic Linkage - genetics
Microsatellite Repeats - genetics
Polymerase Chain Reaction
Polymorphism, Genetic - genetics
Tibet
Tibet
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Summary:The polymorphism distributions of 15 STR loci (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, VWA, TPOX, D18S51, D5S818, and FGA) were investigated in a Tibetan population by multiplex PCR amplification using five fluorochromes (6FAM, VIC, NED, PET, LIZ). Gene frequency, discrimination power (DP), heterozygosity (H), polymorphism information content (PIC) and probability of paternity exclusion (EPP) were calculated, and all loci were tested for Hardy-Weinberg equilibrium. Results indicate that the gene frequency of these 15 STR loci is in Hardy-Weinberg equilibrium. The DP is at 0.7555-0.9602, H is at 0.5651-0.8530, PIC is at 0.5528-0.8456, and EPP is at 0.3811-0.8549. Cumulative DP of the 15 STR is 0.99999999, and cumulative EPP is 0.999999997. Therefore, these 15 STR loci can be used as genetic markers of Tibetan populations in anthropological studies, linkage analysis of genetic diseases, individual identification and paternity testing in forensic medicine.
ISSN:0253-9772
DOI:10.3724/SP.J.1005.2008.00851