New strategy for increasing the specific and efficient expression of transgene in plastids

Plastid gene engineering has become a new way for plant genetic improvements, particularly showing a unique application value in the use of plants as reactors to produce biopharmaceuticals and other important organic compounds. However, plastids only have a semi-autonomous transcription and translat...

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Bibliographic Details
Published inYíchuán Vol. 30; no. 2; p. 142
Main Authors Ren, Qian, Li, Run-Zhi
Format Journal Article
LanguageChinese
Published China 01.02.2008
Subjects
Gene Expression
Genes, Suppressor
Genetic Engineering - methods
Plastids - genetics
Transcriptional Activation
Transgenes - genetics
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Summary:Plastid gene engineering has become a new way for plant genetic improvements, particularly showing a unique application value in the use of plants as reactors to produce biopharmaceuticals and other important organic compounds. However, plastids only have a semi-autonomous transcription and translation machinery. The transcription of endogenous plastid genes is largely dependent on nuclear-encoded transcription factors. Regulation of foreign gene expression in plastids is influenced by various factors. Several technique strategies for regulation of transgene expression in plastids were reported recently, such as hybrid transcription factor-mediated system, phage T7-based transcription system and bacterial lac suppressor-based system. The application and improvement of these systems will greatly enhance the specific and effective expression of the transgenes, and achieve high-level accumulations of foreign proteins in plastids.
ISSN:0253-9772
DOI:10.3724/SP.J.1005.2008.00142