Antitumor properties of nuclear erythroid cells

• Published in: Sibirskiĭ onkologicheskiĭ zhurnal Vol. 21; no. 3; pp. 42 - 49
• Main Authors: Kozlov, V. A., Seledtsova, G. V., Dorzhieva, A. B., Ivanova, I. P., Seledtsov, V. I.
• Format: Journal Article
• Language: English; Russian
• Published: Russian Academy of Sciences, Tomsk National Research Medical Center 29.06.2022
• Subjects: antitumor activity; erythroblasts; nuclear erythroid cells
• ISSN: 1814-4861; 2312-3168
• DOI: 10.21294/1814-4861-2022-21-3-42-49

Purpose. To study suppressor and/or cytotoxic activity of the nuclear erythroid cells (NEC) against tumor cells of various origins. Material and Methods. C57Bl/6 mice and P815, L1210, B16 and L929 tumor cells were used. “Phenylhydrazine” NECs were obtained from mice with induced hemolytic anemia. “Erythropoietin” NECs were isolated from the “phenylhydrazine spleen” and further cultured in the presence of erythropoietin. Another source of NEC was neonatal mouse spleen, human and mouse fetal liver cells, and mouse bone marrow cells cultured with erythropoietin. The cytostatic effect of NEC or their supernatants was recorded by reducing proliferation of P815, L1210, B16, LLC, L929 lines. Results. The presence of pronounced direct antitumor activity was found in both NEC and their culturing products in relation to cells of various tumor lines. The suppressor effect was not specifc. Conclusion. We know about the signifcant numerical predominance of NEC during the embryo development over all other hematopoietic cells and their high suppressive potential. Therefore, it can be assumed that erythroblasts are involved in process of creating antitumor protection of a fetus during this period of life.