Non-tuberculous mycobacteria in phthisiopulmonary practice in the Republic of Uzbekistan

• Published in: Tuberkulëz i bolezni lëgkikh Vol. 99; no. 4; pp. 52 - 56
• Main Authors: Parpieva, N. N., Sultanov, S. A., Dzhurabaeva, M. Kh, Аnvarova, E. V.
• Format: Journal Article
• Language: English; Russian
• Published: New Terra Publishing House 15.05.2021
• Subjects: genotype mycobacterium as/cm; mycobacterium avium сomplex; днк-стрипы; нетуберкулезные микобактерии
• ISSN: 2075-1230; 2542-1506
• DOI: 10.21292/2075-1230-2021-99-4-52-56

The objective of the study : monitoring the spectrum of non-tuberculous mycobacteria isolated from patients who referred for medical care to the Republican Specialized Scientific and Practical Medical Center of Phthisiology and Pulmonology. Subjects and methods . The diagnostic procedure of ATS/IDSA was used to define if the patient suffers from mycobacteriosis. The following specimens were collected to isolate non-tuberculosis mycobacteria: sputum, bronchoalveolar lavage fluid, feces, pleural fluid, surgical and biopsy specimens, and urine. The following tests were performed: Ziehl-Nielsen microscopy, microscopy stained by auramine-O, cultures by Middlebrook 7H9 in BACTEC™ MGIT™ 960 System, Becton Dickinson, USA. Non-tuberculosis mycobateria were differentiated from mycobacterium tuberculosis complex using the sdmpt64 chromatographic test (SD Bioline TBAg MPT64 test, Korea). The non-tuberculosis species were defined by the hybridization technology of DNA* strips GenoType Mycobacterium AS/CM, version 1.0. Results . Of 14,544 patients with suspected respiratory tuberculosis, non-tuberculous mycobacteria were detected in 38 (0.26%) of them, 17 (44.7%) patients had Mycobacterium avium complex, in them there were 26 men (68.4%) and 12 (31.6%) women. Non-tuberculosis mycobacteria were isolated mainly from sputum – in 27 (71.2%) patients and urine – in 6 (15.7%) patients. In 26 (68.4%) patients, mycobacteriosis was caused by slow-growing non-tuberculosis mycobacteria, of which Mycobacterium avium complex prevailed – in 17 people as well as Mgordonae – in 8 patients. Rapidly growing non-tuberculosis mycobacteria were identified in 12 (31.6%) patients, they included M. fortuitum (5 cases) and M. chelonae (4) prevailed.