Brassinolide and [26, 28-2H6]Brassinolide Are Differently Demethylated by Loss of C-26 and C-28, Respectively, inMarchantia polymorpha

• Published in: Plant and cell physiology Vol. 41; no. 10; pp. 1171 - 1174
• Main Authors: Kim, Tae-Wuk, Chang, Soo Chul, Choo, Jongkil, Watanabe, Tsuyoshi, Takatsuto, Suguru, Yokota, Takao, Lee, June Seung, Kim, Soon Young, Kim, Seong-Ki
• Format: Journal Article
• Language: English
• Published: Oxford Oxford Publishing Limited (England) 15.10.2000
• ISSN: 0032-0781; 1471-9053
• DOI: 10.1093/pcp/pcd048

Metabolism of brassinolide in Marchantia polymorpha was investigated by use of in vivo suspension cultured cells. GC-MS analysis of metabolites derived from non-labelled brassinolide and [26, 28-2H6]brassinolide revealed that brassinolide was converted to 26-norbrassinolide while [26, 28-2H6]brassinolide to [26-2H3]28-norbrassinolide. It seems that Marchantia cells recognized [26, 28-2H6]brassinolide as a xenobiotic rather than brassinolide and deteriums attached to C-28 significantly affect demethylation reaction due to isotopic effect. Thus, demethylation of brassinolide in planta seems to proceed by loss of C-26 rather than C-28. The present finding is the first evidence for demethylation metabolism of brassinosteroids. The biological activity of 26-norbrassinolide was 10-fold reduced as shown by the rice lamina inclination test. However, because of its high biological activity, it remains difficult to conclude whether or not C-26 demethylation serves as an important deactivation process of brassinolide.