Expression of TNF, IL1B, and  iNOS2 in the neural cell after induced by Porphyromonas gingivalis with and without coating antibody anti -Porphyromonas gingivalis [version 3; peer review: 1 approved, 1 approved with reservations]

Porphyromonas gingivalis has virulence factors such as gingipain and lipopolysaccharide, causing bacteremia to reach the brain and activate neuroinflammatory release cytokines. This study analyzed the effect of the co-culture of neuron cells with P. gingivalis coated with anti -P. gingivalis antibod...

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Bibliographic Details
Published inF1000 research Vol. 9; p. 1499
Main Authors Bachtiar, Endang Winiati, Putri, Citra F, Soejoedono, Retno D, Bachtiar, Boy M
Format Journal Article
LanguageEnglish
Published England 2020
Subjects
Antibodies
Gingipain Cysteine Endopeptidases
Lipopolysaccharides
Neurons
Porphyromonas gingivalis
Porphyromonas gingivalis
Blocking Antibody
Neuroinflammation
iNOS
TNF-α
IL-1β
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Summary:Porphyromonas gingivalis has virulence factors such as gingipain and lipopolysaccharide, causing bacteremia to reach the brain and activate neuroinflammatory release cytokines. This study analyzed the effect of the co-culture of neuron cells with P. gingivalis coated with anti -P. gingivalis antibodies against cytokines produced by neuron cells. The gene expressions of the TNF, IL1B,  iNOS2 in neurons was evaluated using RT-qPCR. The results showed that P. gingivalis coated with anti -P. gingivalis antibody before co-culture with neuron cells could decrease the gene expression of TNF, IL1B, and  iNOS2 of neuron cells.
Bibliography:new_version
ISSN:2046-1402
2046-1402
DOI:10.12688/f1000research.26749.3