Effect of pyruvate on human sperm in vitro

The study objective is to investigate the biological effect of energy substrate (pyruvate) on human sperm motility in vitro.Materials and methods. The study was conducted using human sperm from 14 ejaculate samples obtained by the standard method. Motility and viability were evaluated per the World...

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Published inAndrologii͡a︡ i genitalʹnai͡a︡ khirurgii͡a Vol. 21; no. 3; pp. 56 - 60
Main Authors Evdokimov, V. V., Isaev, N. K., Turovetsky, V. B.
Format Journal Article
LanguageEnglish
Russian
Published ABV-press 15.11.2020
Subjects
experiment in vitro
motility
pyruvate
spermatozoa
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Summary:The study objective is to investigate the biological effect of energy substrate (pyruvate) on human sperm motility in vitro.Materials and methods. The study was conducted using human sperm from 14 ejaculate samples obtained by the standard method. Motility and viability were evaluated per the World Health Organization guidelines under 400x magnification. After dilution, 1 ml was sampled from the ejaculate for the experiment and control. The experiment consisted of addition of pyruvate to the test tube with the ejaculate in final concentration of 1 mmol.Results. The level of initial sperm motility corresponded to normozoospermia. The percentage of progressive motile sperm increased by 24, 67 and 84 % after 30, 60 and 120 min of the experiment. The percentage of non-progressive motile sperm and total percentage of motile sperm showed no significant change during this time period. The number of live spermatozoa showed no significant change during the experiment.Conclusion. The study showed that pyruvate can stimulate motility of human sperm in vitro.
ISSN:2070-9781
2412-8902
DOI:10.17650/2070-9781-2020-21-3-56-60