Biosensors based on the luminous bacteria Photobaterium phosphoreum immobilized in polyvinyl alcohol cryogel for the monitoring of ecotoxicants

Immobilization of Photobacterium phosphoreum bacteria in polyvinyl alcohol cryogel was performed in order to develop biosensors used for ecotoxicant biomonitoring. The immobilization procedure, storage, and application of the immobilized cells for biomonitoring were optimized. It was shown that the...

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Bibliographic Details
Published inPrikladnaja biohimija i mikrobiologija Vol. 50; no. 5; p. 490
Main Authors Efremenko, E N, Sen'ko, O V, Aleskerova, L É, Alenina, K A, Mazhul', M M, Ismailov, A D
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.09.2014
Subjects
Biosensing Techniques - instrumentation
Biosensing Techniques - methods
Cells, Immobilized
Chlorophenols - analysis
Cryogels
Environmental Monitoring
Luminescent Measurements
Metals, Heavy - analysis
Photobacterium - chemistry
Photobacterium - metabolism
Polyvinyl Alcohol - chemistry
Time Factors
Water Pollutants, Chemical - analysis
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Summary:Immobilization of Photobacterium phosphoreum bacteria in polyvinyl alcohol cryogel was performed in order to develop biosensors used for ecotoxicant biomonitoring. The immobilization procedure, storage, and application of the immobilized cells for biomonitoring were optimized. It was shown that the immobilized cells demonstrate significantly higher stability and a longer duration of light emission than free bacteria. A discrete analysis of heavy metals and chlorophenols was conducted using the obtained biosensor samples.
ISSN:0555-1099
DOI:10.7868/S0555109914050031