Improvement of Heat Shock Treatment and Trial Development of Hot Water Sprayer for Inducing Disease Resistance in Cucumber (Cucumis sativus L.)

• Published in: Engeigaku kenkyuu Vol. 11; no. 1; pp. 121 - 126
• Main Authors: Yoshino, Mioko, Widiastuti, Ani, Songying, Zhou, Odani, Hiromitsu, Hasegawa, Morifumi, Sato, Tatsuo
• Format: Journal Article
• Language: Japanese
• Published: THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE 2012
• Subjects: hot water dipping; hot water spray; peroxidase; salicylic acid; systemic acquired resistance
• ISSN: 1347-2658; 1880-3571
• DOI: 10.2503/hrj.11.121

This research was conducted to establish a practical method of heat shock treatment to induce disease resistance in cucumber seedlings. To achieve this purpose, short treatment at a higher temperature than in our previous report was tested and a prototype hot water sprayer was developed for treatment. At a result of the hot water dipping test, treatment at 50°C for 20 seconds showed the same effect as 40°C for 120 seconds, which was the optimum condition in the previous experiment for inducing resistance to gray mold (Botritis cinerea). Based on this result, a prototype hot water sprayer was assembled using an LP gas boiler as the main component. A thermal cover was effective for maintaining leaf temperature at 50°C stably. When hot water was used to treat the leaves appropriately using this machine, salicylic acid concentration and the expression level of the peroxidase gene increased in the leaves. An inoculation test of gray mold showed the same protective effect as treatment with saccharine sodium, known as a plant activator. As a result, we concluded that the hot water spray is a possible practical method for inducing disease resistance to grey mold in cucumber seedlings.