Functional complementation of a conserved non protein-coding element (CNE) of Autographa californica multiple nucleopolyhedrovirus by heterologous CNE originating from Malacosoma neustria nucleopolyhedrovirus

Aim. To assess the functional conservation of a baculovirus CNE. Methods. CNE deletion from the baculovirus genome and its replacement with a heterologous CNE was performed by using the bacmid-based technology. Transfection-infection assays were used to investigate cell-to-cell spread of the recombi...

Full description

Saved in:
Bibliographic Details
Published inBiopolimery i kletka Vol. 32; no. 3; pp. 173 - 178
Main Authors Kikhno, I. M., Makarenko, V. E., Kashuba, V. I.
Format Journal Article
LanguageEnglish
Published Kiev Natsional'na Akademiya Nauk Ukrainy - National Academy of Sciences of Ukraine 2016
Subjects
Autographa californica
Cell culture
Clonal deletion
Complementation
Genomes
Malacosoma neustria
Proteins
Transfection
Online AccessGet full text

Cover

More Information
Summary:Aim. To assess the functional conservation of a baculovirus CNE. Methods. CNE deletion from the baculovirus genome and its replacement with a heterologous CNE was performed by using the bacmid-based technology. Transfection-infection assays were used to investigate cell-to-cell spread of the recombinant virus. Results. The recombinant Autographa californica multiple nucleopolyhedrovirus genome carrying the Malacosoma neustria nuclepolyhedrovirus CNE in place of the innate CNE was constructed. The recombinant virus was able to transmit the infection in a cell culture. Conclusions . The CNE essential function was foundto be conserved between the representatives of I and II groups of the genus Alphabaculovirus.
ISSN:0233-7657
1993-6842
DOI:10.7124/bc.00091B