Immunohistochemical Detection of Coxiella burnetii in Cattle Spleen Organs from Ampel Slaughterhouse, Boyolali Regency

Q-fever is a zoonotic bacterial disease that caused by Coxiella burnetii. These microorganism are gram negative and obligate intracellular bacteria. This study was conducted to detect C. burnetii in cattle organs which collected from Ampel slaughterhouse Boyolali Regency. In this study, spleen, hear...

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Bibliographic Details
Published inJurnal Medik Veteriner Vol. 4; no. 1; pp. 48 - 55
Main Authors Nugroho, Eko Prasetyo, Setiyono, Agus, Hadi, Upik Kesumawati, Winarsih, Wiwin, Astuti, Dwi
Format Journal Article
LanguageEnglish
Published Universitas Airlangga, Faculty of Veterinary Medicine 15.04.2021
Subjects
boyolali
cattle
coxiella burnetii
immunohistochemical
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Summary:Q-fever is a zoonotic bacterial disease that caused by Coxiella burnetii. These microorganism are gram negative and obligate intracellular bacteria. This study was conducted to detect C. burnetii in cattle organs which collected from Ampel slaughterhouse Boyolali Regency. In this study, spleen, heart, lung, liver and kidney were collected from 100 cattle. The samples were tested by immunohistochemical (IHC) method using polyclonal anti- C. burnetii antibodies. Immunohistochemical examination found the presence of C. burnetii in the cytoplasm of macrophage cells with specific brown color only in the spleen as many as 4 out of 100 cattle showing immunoreactive (4%). The four positive individual samples were from Simental cattle. These results indicate that Q-fever was found in local cattle in Boyolali Regency.
ISSN:2615-7497
2581-012X
DOI:10.20473/jmv.vol4.iss1.2021.48-55