Investigating the relationship between ccfDNA concentration, its integrity, and some individual factors in an Iranian population

• Published in: Human antibodies Vol. 28; no. 4; pp. 319 - 326
• Main Authors: Khani, Maryam, Hosseini, Jalil, Habibi, Mohsen, Mirfakhraie, Reza, Sadeghzadeh, Zahra, Pouresmaeili, Farkhondeh
• Format: Journal Article
• Language: English
• Published: Amsterdam IOS Press BV 2020
• Subjects: Biomarkers; Deoxyribonucleic acid; Disease; DNA; Family medical history; Genetics; Hyperplasia; Integrity; Peripheral blood; Prostate cancer; Statistics; Weight
• ISSN: 1093-2607; 1875-869X
• DOI: 10.3233/HAB-200419

INTRODUCTION: Circulating cell-free DNA (ccfDNA) increases in some pathologic conditions like cancer. We aimed to investigate the correlation between some individual factors and the ccfDNA level in peripheral blood of Iranian in relation to prostate cancer. MATERIAL AND METHOD: 30 patients with prostate cancer (PCa), 40 with benign prostate hyperplasia (BPH), and 30 controls were studied. Personal information, ccfDNA concentration, and the integrity index were assessed for the correlation between the disease and different factors. The results were statistically analyzed using SPSS software. RESULTS: In PCa group, no association was found between total ccfDNA, BMI, BPH background, non-cancerous diseases, medications, PCa length, and job (p-value > 0.05). But, total ccfDNA had statistical associations with weight, family history of cancer, and location (p-value < 0.05). No association was between the integrity of ccfDNA, weight, the background of BPH, and family history of cancer. But, the integrity of ccfDNA was significantly associated with BMI and PCa length (p-value < 0.05). In BPH group, no association between total ccfDNA or the integrity of ccfDNA and the assessed factors was obtained (p-value > 0.05). In the normal group, neither statistical association was found between total ccfDNA, weight, BMI, and job, nor between the integrity of ccfDNA, weight, BMI, non-cancerous disease, drug, job, and location (p-value > 0.05). But, a statistical association was found between the integrity of ccfDNA and family history of cancer in the recent group (Based on 95% CI and P-value less than 0.05). CONCLUSION: ccfDNA and its integrity as possible prostate cancer biomarkers under the influence of individuals’ physiological status are prone to the pathologic changes toward the disease. Further simultaneous study of the target groups could clarify this matter.