Molecular Characterization and DNA Fingerprinting of Xanthomonas oryzae pv. oryzae Isolates from Climate Change Prone Areas in East Africa

• Published in: Research journal of environmental sciences Vol. 5; no. 9; pp. 772 - 778
• Main Authors: Onasanya, A., Kiepe, P., Basso, A., Nkima, G., Nwilene, F.E., Ingelbrech, I., Lamo, J., Ekperigin, M.M., Onasanya, R.O., Oyelakin, O., Winter, S., Sere, Y.
• Format: Journal Article
• Language: English
• Published: 01.09.2011
• Subjects: Oryza sativa; Xanthomonas oryzae; Uganda; Philippines; Africa; Rwanda
• ISSN: 1819-3412
• DOI: 10.3923/rjes.2011.772.778

Genomic DNA fingerprinting is a useful tool for effective and reliable identification and differentiation of Xanthomonas oryzae pv. oryzae (Xoo) pathogen from rice. The study aimed to conduct molecular characterization and DNA fingerprinting of 23 Xoo isolates from East Africa and two Xoo isolates from IRRI (Philippines) as control. PCR analysis was carryout on genomic DNA of 25 Xoo isolates using 6 Xoo specific primer pairs. Cluster analyses of genetic data obtained from 25 Xoo DNA fingerprints revealed two major genotypes (GrpA and GrpB) among the 25 Xoo isolates. Some Xoo isolates from Rwanda and Uganda were identical suggesting possible pathogen migration between these countries and long-term survival. Durable resistance rice cultivars would need to overcome both GrpA and GrpB Xoo genotypes in order to survive after their deployment into different rice ecologies in East Africa.